Coding

Part:BBa_K1361997:Design

Designed by: Shoujie Sun   Group: iGEM14_Tianjin   (2014-10-03)


CsgB, curlin nucleator protein, minor subunit in curli complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CsgB can be designed under inductively control while make CsgACEFG genes constitutively express respectly. Using this method could achieve the immediate synthesis of curli fiber after inducer has been added.


Source

PCR on genomic DNA from E coli. DH5alpha strain.

References